PoplarV1, Main, Exploration, bibRecord, 001800

Knockdown of a laccase in Populus deltoides confers altered cell wall chemistry and increased sugar release.

Identifieur interne : 001800 ( Main/Exploration ); précédent : 001799; suivant : 001801

Knockdown of a laccase in Populus deltoides confers altered cell wall chemistry and increased sugar release.

Auteurs : Anthony C. Bryan [États-Unis] ; Sara Jawdy [États-Unis] ; Lee Gunter [États-Unis] ; Erica Gjersing [États-Unis] ; Robert Sykes [États-Unis] ; Maud A W. Hinchee [États-Unis] ; Kimberly A. Winkeler [États-Unis] ; Cassandra M. Collins [États-Unis] ; Nancy Engle [États-Unis] ; Timothy J. Tschaplinski [États-Unis] ; Xiaohan Yang [États-Unis] ; Gerald A. Tuskan [États-Unis] ; Wellington Muchero [États-Unis] ; Jin-Gui Chen [États-Unis]

Source :

Plant biotechnology journal [ 1467-7652 ] ; 2016.

RBID : pubmed:26997157

Descripteurs français

KwdFr :
- Laccase (génétique), Laccase (métabolisme), Lignine (métabolisme), Paroi cellulaire (composition chimique), Paroi cellulaire (enzymologie), Paroi cellulaire (génétique), Populus (enzymologie), Populus (génétique), Régulation de l'expression des gènes végétaux (génétique), Végétaux génétiquement modifiés (enzymologie), Végétaux génétiquement modifiés (génétique), Xylose (métabolisme).
MESH :
- composition chimique : Paroi cellulaire.
- enzymologie : Paroi cellulaire, Populus, Végétaux génétiquement modifiés.
- génétique : Laccase, Paroi cellulaire, Populus, Régulation de l'expression des gènes végétaux, Végétaux génétiquement modifiés.
- métabolisme : Laccase, Lignine, Xylose.

English descriptors

KwdEn :
- Cell Wall (chemistry), Cell Wall (enzymology), Cell Wall (genetics), Gene Expression Regulation, Plant (genetics), Laccase (genetics), Laccase (metabolism), Lignin (metabolism), Plants, Genetically Modified (enzymology), Plants, Genetically Modified (genetics), Populus (enzymology), Populus (genetics), Xylose (metabolism).
MESH :
- chemical , genetics : Laccase.
- chemistry : Cell Wall.
- enzymology : Cell Wall, Plants, Genetically Modified, Populus.
- genetics : Cell Wall, Gene Expression Regulation, Plant, Plants, Genetically Modified, Populus.
- chemical , metabolism : Laccase, Lignin, Xylose.

Abstract

Plant laccases are thought to function in the oxidation of monolignols which leads to higher order lignin formation. Only a hand-full of laccases in plants have been functionally evaluated, and as such little is known about the breadth of their impact on cell wall chemistry or structure. Here, we describe a previously uncharacterized laccase from Populus, encoded by locus Potri.008G064000, whose reduced expression resulted in transgenic Populus trees with changes in syringyl/guaiacyl ratios as well as altered sugar release phenotypes. These phenotypes are consistent with plant biomass exhibiting reduced recalcitrance. Interestingly, the transgene effect on recalcitrance is dependent on a mild pretreatment prior to chemical extraction of sugars. Metabolite profiling suggests the transgene modulates phenolics that are associated with the cell wall structure. We propose that this particular laccase has a range of functions related to oxidation of phenolics and conjugation of flavonoids that interact with lignin in the cell wall.

DOI: 10.1111/pbi.12560
PubMed: 26997157
PubMed Central: PMC5043505

Affiliations:

Links toward previous steps (curation, corpus...)

to stream Main, to step Corpus: 001863
to stream Main, to step Curation: 001863

Le document en format XML

<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Knockdown of a laccase in Populus deltoides confers altered cell wall chemistry and increased sugar release.</title>
<author><name sortKey="Bryan, Anthony C" sort="Bryan, Anthony C" uniqKey="Bryan A" first="Anthony C" last="Bryan">Anthony C. Bryan</name>
<affiliation wicri:level="2"><nlm:affiliation>BioEnergy Science Center and Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>BioEnergy Science Center and Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN</wicri:regionArea>
<placeName><region type="state">Tennessee</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Jawdy, Sara" sort="Jawdy, Sara" uniqKey="Jawdy S" first="Sara" last="Jawdy">Sara Jawdy</name>
<affiliation wicri:level="2"><nlm:affiliation>BioEnergy Science Center and Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>BioEnergy Science Center and Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN</wicri:regionArea>
<placeName><region type="state">Tennessee</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Gunter, Lee" sort="Gunter, Lee" uniqKey="Gunter L" first="Lee" last="Gunter">Lee Gunter</name>
<affiliation wicri:level="2"><nlm:affiliation>BioEnergy Science Center and Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>BioEnergy Science Center and Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN</wicri:regionArea>
<placeName><region type="state">Tennessee</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Gjersing, Erica" sort="Gjersing, Erica" uniqKey="Gjersing E" first="Erica" last="Gjersing">Erica Gjersing</name>
<affiliation wicri:level="2"><nlm:affiliation>The Biosciences Center, National Renewable Energy Laboratory, Golden, CO, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>The Biosciences Center, National Renewable Energy Laboratory, Golden, CO</wicri:regionArea>
<placeName><region type="state">Colorado</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Sykes, Robert" sort="Sykes, Robert" uniqKey="Sykes R" first="Robert" last="Sykes">Robert Sykes</name>
<affiliation wicri:level="2"><nlm:affiliation>The Biosciences Center, National Renewable Energy Laboratory, Golden, CO, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>The Biosciences Center, National Renewable Energy Laboratory, Golden, CO</wicri:regionArea>
<placeName><region type="state">Colorado</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Hinchee, Maud A W" sort="Hinchee, Maud A W" uniqKey="Hinchee M" first="Maud A W" last="Hinchee">Maud A W. Hinchee</name>
<affiliation wicri:level="2"><nlm:affiliation>ArborGen Inc, Ridgeville, SC, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>ArborGen Inc, Ridgeville, SC</wicri:regionArea>
<placeName><region type="state">Caroline du Sud</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Winkeler, Kimberly A" sort="Winkeler, Kimberly A" uniqKey="Winkeler K" first="Kimberly A" last="Winkeler">Kimberly A. Winkeler</name>
<affiliation wicri:level="2"><nlm:affiliation>ArborGen Inc, Ridgeville, SC, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>ArborGen Inc, Ridgeville, SC</wicri:regionArea>
<placeName><region type="state">Caroline du Sud</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Collins, Cassandra M" sort="Collins, Cassandra M" uniqKey="Collins C" first="Cassandra M" last="Collins">Cassandra M. Collins</name>
<affiliation wicri:level="2"><nlm:affiliation>ArborGen Inc, Ridgeville, SC, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>ArborGen Inc, Ridgeville, SC</wicri:regionArea>
<placeName><region type="state">Caroline du Sud</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Engle, Nancy" sort="Engle, Nancy" uniqKey="Engle N" first="Nancy" last="Engle">Nancy Engle</name>
<affiliation wicri:level="2"><nlm:affiliation>BioEnergy Science Center and Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>BioEnergy Science Center and Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN</wicri:regionArea>
<placeName><region type="state">Tennessee</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Tschaplinski, Timothy J" sort="Tschaplinski, Timothy J" uniqKey="Tschaplinski T" first="Timothy J" last="Tschaplinski">Timothy J. Tschaplinski</name>
<affiliation wicri:level="2"><nlm:affiliation>BioEnergy Science Center and Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>BioEnergy Science Center and Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN</wicri:regionArea>
<placeName><region type="state">Tennessee</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Yang, Xiaohan" sort="Yang, Xiaohan" uniqKey="Yang X" first="Xiaohan" last="Yang">Xiaohan Yang</name>
<affiliation wicri:level="2"><nlm:affiliation>BioEnergy Science Center and Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>BioEnergy Science Center and Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN</wicri:regionArea>
<placeName><region type="state">Tennessee</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Tuskan, Gerald A" sort="Tuskan, Gerald A" uniqKey="Tuskan G" first="Gerald A" last="Tuskan">Gerald A. Tuskan</name>
<affiliation wicri:level="2"><nlm:affiliation>BioEnergy Science Center and Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>BioEnergy Science Center and Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN</wicri:regionArea>
<placeName><region type="state">Tennessee</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Muchero, Wellington" sort="Muchero, Wellington" uniqKey="Muchero W" first="Wellington" last="Muchero">Wellington Muchero</name>
<affiliation wicri:level="2"><nlm:affiliation>BioEnergy Science Center and Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, USA. mucherow@ornl.gov.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>BioEnergy Science Center and Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN</wicri:regionArea>
<placeName><region type="state">Tennessee</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Chen, Jin Gui" sort="Chen, Jin Gui" uniqKey="Chen J" first="Jin-Gui" last="Chen">Jin-Gui Chen</name>
<affiliation wicri:level="2"><nlm:affiliation>BioEnergy Science Center and Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, USA. chenj@ornl.gov.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>BioEnergy Science Center and Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN</wicri:regionArea>
<placeName><region type="state">Tennessee</region>
</placeName>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PubMed</idno>
<date when="2016">2016</date>
<idno type="RBID">pubmed:26997157</idno>
<idno type="pmid">26997157</idno>
<idno type="pmc">PMC5043505</idno>
<idno type="doi">10.1111/pbi.12560</idno>
<idno type="wicri:Area/Main/Corpus">001863</idno>
<idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">001863</idno>
<idno type="wicri:Area/Main/Curation">001863</idno>
<idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">001863</idno>
<idno type="wicri:Area/Main/Exploration">001863</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">Knockdown of a laccase in Populus deltoides confers altered cell wall chemistry and increased sugar release.</title>
<author><name sortKey="Bryan, Anthony C" sort="Bryan, Anthony C" uniqKey="Bryan A" first="Anthony C" last="Bryan">Anthony C. Bryan</name>
<affiliation wicri:level="2"><nlm:affiliation>BioEnergy Science Center and Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>BioEnergy Science Center and Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN</wicri:regionArea>
<placeName><region type="state">Tennessee</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Jawdy, Sara" sort="Jawdy, Sara" uniqKey="Jawdy S" first="Sara" last="Jawdy">Sara Jawdy</name>
<affiliation wicri:level="2"><nlm:affiliation>BioEnergy Science Center and Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>BioEnergy Science Center and Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN</wicri:regionArea>
<placeName><region type="state">Tennessee</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Gunter, Lee" sort="Gunter, Lee" uniqKey="Gunter L" first="Lee" last="Gunter">Lee Gunter</name>
<affiliation wicri:level="2"><nlm:affiliation>BioEnergy Science Center and Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>BioEnergy Science Center and Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN</wicri:regionArea>
<placeName><region type="state">Tennessee</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Gjersing, Erica" sort="Gjersing, Erica" uniqKey="Gjersing E" first="Erica" last="Gjersing">Erica Gjersing</name>
<affiliation wicri:level="2"><nlm:affiliation>The Biosciences Center, National Renewable Energy Laboratory, Golden, CO, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>The Biosciences Center, National Renewable Energy Laboratory, Golden, CO</wicri:regionArea>
<placeName><region type="state">Colorado</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Sykes, Robert" sort="Sykes, Robert" uniqKey="Sykes R" first="Robert" last="Sykes">Robert Sykes</name>
<affiliation wicri:level="2"><nlm:affiliation>The Biosciences Center, National Renewable Energy Laboratory, Golden, CO, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>The Biosciences Center, National Renewable Energy Laboratory, Golden, CO</wicri:regionArea>
<placeName><region type="state">Colorado</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Hinchee, Maud A W" sort="Hinchee, Maud A W" uniqKey="Hinchee M" first="Maud A W" last="Hinchee">Maud A W. Hinchee</name>
<affiliation wicri:level="2"><nlm:affiliation>ArborGen Inc, Ridgeville, SC, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>ArborGen Inc, Ridgeville, SC</wicri:regionArea>
<placeName><region type="state">Caroline du Sud</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Winkeler, Kimberly A" sort="Winkeler, Kimberly A" uniqKey="Winkeler K" first="Kimberly A" last="Winkeler">Kimberly A. Winkeler</name>
<affiliation wicri:level="2"><nlm:affiliation>ArborGen Inc, Ridgeville, SC, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>ArborGen Inc, Ridgeville, SC</wicri:regionArea>
<placeName><region type="state">Caroline du Sud</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Collins, Cassandra M" sort="Collins, Cassandra M" uniqKey="Collins C" first="Cassandra M" last="Collins">Cassandra M. Collins</name>
<affiliation wicri:level="2"><nlm:affiliation>ArborGen Inc, Ridgeville, SC, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>ArborGen Inc, Ridgeville, SC</wicri:regionArea>
<placeName><region type="state">Caroline du Sud</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Engle, Nancy" sort="Engle, Nancy" uniqKey="Engle N" first="Nancy" last="Engle">Nancy Engle</name>
<affiliation wicri:level="2"><nlm:affiliation>BioEnergy Science Center and Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>BioEnergy Science Center and Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN</wicri:regionArea>
<placeName><region type="state">Tennessee</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Tschaplinski, Timothy J" sort="Tschaplinski, Timothy J" uniqKey="Tschaplinski T" first="Timothy J" last="Tschaplinski">Timothy J. Tschaplinski</name>
<affiliation wicri:level="2"><nlm:affiliation>BioEnergy Science Center and Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>BioEnergy Science Center and Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN</wicri:regionArea>
<placeName><region type="state">Tennessee</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Yang, Xiaohan" sort="Yang, Xiaohan" uniqKey="Yang X" first="Xiaohan" last="Yang">Xiaohan Yang</name>
<affiliation wicri:level="2"><nlm:affiliation>BioEnergy Science Center and Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>BioEnergy Science Center and Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN</wicri:regionArea>
<placeName><region type="state">Tennessee</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Tuskan, Gerald A" sort="Tuskan, Gerald A" uniqKey="Tuskan G" first="Gerald A" last="Tuskan">Gerald A. Tuskan</name>
<affiliation wicri:level="2"><nlm:affiliation>BioEnergy Science Center and Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>BioEnergy Science Center and Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN</wicri:regionArea>
<placeName><region type="state">Tennessee</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Muchero, Wellington" sort="Muchero, Wellington" uniqKey="Muchero W" first="Wellington" last="Muchero">Wellington Muchero</name>
<affiliation wicri:level="2"><nlm:affiliation>BioEnergy Science Center and Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, USA. mucherow@ornl.gov.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>BioEnergy Science Center and Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN</wicri:regionArea>
<placeName><region type="state">Tennessee</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Chen, Jin Gui" sort="Chen, Jin Gui" uniqKey="Chen J" first="Jin-Gui" last="Chen">Jin-Gui Chen</name>
<affiliation wicri:level="2"><nlm:affiliation>BioEnergy Science Center and Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, USA. chenj@ornl.gov.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>BioEnergy Science Center and Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN</wicri:regionArea>
<placeName><region type="state">Tennessee</region>
</placeName>
</affiliation>
</author>
</analytic>
<series><title level="j">Plant biotechnology journal</title>
<idno type="eISSN">1467-7652</idno>
<imprint><date when="2016" type="published">2016</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Cell Wall (chemistry)</term>
<term>Cell Wall (enzymology)</term>
<term>Cell Wall (genetics)</term>
<term>Gene Expression Regulation, Plant (genetics)</term>
<term>Laccase (genetics)</term>
<term>Laccase (metabolism)</term>
<term>Lignin (metabolism)</term>
<term>Plants, Genetically Modified (enzymology)</term>
<term>Plants, Genetically Modified (genetics)</term>
<term>Populus (enzymology)</term>
<term>Populus (genetics)</term>
<term>Xylose (metabolism)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Laccase (génétique)</term>
<term>Laccase (métabolisme)</term>
<term>Lignine (métabolisme)</term>
<term>Paroi cellulaire (composition chimique)</term>
<term>Paroi cellulaire (enzymologie)</term>
<term>Paroi cellulaire (génétique)</term>
<term>Populus (enzymologie)</term>
<term>Populus (génétique)</term>
<term>Régulation de l'expression des gènes végétaux (génétique)</term>
<term>Végétaux génétiquement modifiés (enzymologie)</term>
<term>Végétaux génétiquement modifiés (génétique)</term>
<term>Xylose (métabolisme)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Laccase</term>
</keywords>
<keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Cell Wall</term>
</keywords>
<keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Paroi cellulaire</term>
</keywords>
<keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Paroi cellulaire</term>
<term>Populus</term>
<term>Végétaux génétiquement modifiés</term>
</keywords>
<keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Cell Wall</term>
<term>Plants, Genetically Modified</term>
<term>Populus</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Cell Wall</term>
<term>Gene Expression Regulation, Plant</term>
<term>Plants, Genetically Modified</term>
<term>Populus</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Laccase</term>
<term>Paroi cellulaire</term>
<term>Populus</term>
<term>Régulation de l'expression des gènes végétaux</term>
<term>Végétaux génétiquement modifiés</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Laccase</term>
<term>Lignin</term>
<term>Xylose</term>
</keywords>
<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Laccase</term>
<term>Lignine</term>
<term>Xylose</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Plant laccases are thought to function in the oxidation of monolignols which leads to higher order lignin formation. Only a hand-full of laccases in plants have been functionally evaluated, and as such little is known about the breadth of their impact on cell wall chemistry or structure. Here, we describe a previously uncharacterized laccase from Populus, encoded by locus Potri.008G064000, whose reduced expression resulted in transgenic Populus trees with changes in syringyl/guaiacyl ratios as well as altered sugar release phenotypes. These phenotypes are consistent with plant biomass exhibiting reduced recalcitrance. Interestingly, the transgene effect on recalcitrance is dependent on a mild pretreatment prior to chemical extraction of sugars. Metabolite profiling suggests the transgene modulates phenolics that are associated with the cell wall structure. We propose that this particular laccase has a range of functions related to oxidation of phenolics and conjugation of flavonoids that interact with lignin in the cell wall.</div>
</front>
</TEI>
<pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">26997157</PMID>
<DateCompleted><Year>2017</Year>
<Month>06</Month>
<Day>01</Day>
</DateCompleted>
<DateRevised><Year>2018</Year>
<Month>11</Month>
<Day>13</Day>
</DateRevised>
<Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1467-7652</ISSN>
<JournalIssue CitedMedium="Internet"><Volume>14</Volume>
<Issue>10</Issue>
<PubDate><Year>2016</Year>
<Month>10</Month>
</PubDate>
</JournalIssue>
<Title>Plant biotechnology journal</Title>
<ISOAbbreviation>Plant Biotechnol J</ISOAbbreviation>
</Journal>
<ArticleTitle>Knockdown of a laccase in Populus deltoides confers altered cell wall chemistry and increased sugar release.</ArticleTitle>
<Pagination><MedlinePgn>2010-20</MedlinePgn>
</Pagination>
<ELocationID EIdType="doi" ValidYN="Y">10.1111/pbi.12560</ELocationID>
<Abstract><AbstractText>Plant laccases are thought to function in the oxidation of monolignols which leads to higher order lignin formation. Only a hand-full of laccases in plants have been functionally evaluated, and as such little is known about the breadth of their impact on cell wall chemistry or structure. Here, we describe a previously uncharacterized laccase from Populus, encoded by locus Potri.008G064000, whose reduced expression resulted in transgenic Populus trees with changes in syringyl/guaiacyl ratios as well as altered sugar release phenotypes. These phenotypes are consistent with plant biomass exhibiting reduced recalcitrance. Interestingly, the transgene effect on recalcitrance is dependent on a mild pretreatment prior to chemical extraction of sugars. Metabolite profiling suggests the transgene modulates phenolics that are associated with the cell wall structure. We propose that this particular laccase has a range of functions related to oxidation of phenolics and conjugation of flavonoids that interact with lignin in the cell wall.</AbstractText>
<CopyrightInformation>© 2016 The Authors. Plant Biotechnology Journal published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd.</CopyrightInformation>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Bryan</LastName>
<ForeName>Anthony C</ForeName>
<Initials>AC</Initials>
<Identifier Source="ORCID">0000-0003-0917-8705</Identifier>
<AffiliationInfo><Affiliation>BioEnergy Science Center and Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Jawdy</LastName>
<ForeName>Sara</ForeName>
<Initials>S</Initials>
<AffiliationInfo><Affiliation>BioEnergy Science Center and Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Gunter</LastName>
<ForeName>Lee</ForeName>
<Initials>L</Initials>
<AffiliationInfo><Affiliation>BioEnergy Science Center and Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Gjersing</LastName>
<ForeName>Erica</ForeName>
<Initials>E</Initials>
<AffiliationInfo><Affiliation>The Biosciences Center, National Renewable Energy Laboratory, Golden, CO, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Sykes</LastName>
<ForeName>Robert</ForeName>
<Initials>R</Initials>
<AffiliationInfo><Affiliation>The Biosciences Center, National Renewable Energy Laboratory, Golden, CO, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Hinchee</LastName>
<ForeName>Maud A W</ForeName>
<Initials>MA</Initials>
<AffiliationInfo><Affiliation>ArborGen Inc, Ridgeville, SC, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Winkeler</LastName>
<ForeName>Kimberly A</ForeName>
<Initials>KA</Initials>
<AffiliationInfo><Affiliation>ArborGen Inc, Ridgeville, SC, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Collins</LastName>
<ForeName>Cassandra M</ForeName>
<Initials>CM</Initials>
<AffiliationInfo><Affiliation>ArborGen Inc, Ridgeville, SC, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Engle</LastName>
<ForeName>Nancy</ForeName>
<Initials>N</Initials>
<AffiliationInfo><Affiliation>BioEnergy Science Center and Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Tschaplinski</LastName>
<ForeName>Timothy J</ForeName>
<Initials>TJ</Initials>
<AffiliationInfo><Affiliation>BioEnergy Science Center and Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Yang</LastName>
<ForeName>Xiaohan</ForeName>
<Initials>X</Initials>
<AffiliationInfo><Affiliation>BioEnergy Science Center and Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Tuskan</LastName>
<ForeName>Gerald A</ForeName>
<Initials>GA</Initials>
<AffiliationInfo><Affiliation>BioEnergy Science Center and Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Muchero</LastName>
<ForeName>Wellington</ForeName>
<Initials>W</Initials>
<AffiliationInfo><Affiliation>BioEnergy Science Center and Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, USA. mucherow@ornl.gov.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Chen</LastName>
<ForeName>Jin-Gui</ForeName>
<Initials>JG</Initials>
<AffiliationInfo><Affiliation>BioEnergy Science Center and Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, USA. chenj@ornl.gov.</Affiliation>
</AffiliationInfo>
</Author>
</AuthorList>
<Language>eng</Language>
<DataBankList CompleteYN="Y"><DataBank><DataBankName>GENBANK</DataBankName>
<AccessionNumberList><AccessionNumber>KP259613</AccessionNumber>
</AccessionNumberList>
</DataBank>
</DataBankList>
<PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType>
<PublicationType UI="D013486">Research Support, U.S. Gov't, Non-P.H.S.</PublicationType>
</PublicationTypeList>
<ArticleDate DateType="Electronic"><Year>2016</Year>
<Month>04</Month>
<Day>15</Day>
</ArticleDate>
</Article>
<MedlineJournalInfo><Country>England</Country>
<MedlineTA>Plant Biotechnol J</MedlineTA>
<NlmUniqueID>101201889</NlmUniqueID>
<ISSNLinking>1467-7644</ISSNLinking>
</MedlineJournalInfo>
<ChemicalList><Chemical><RegistryNumber>9005-53-2</RegistryNumber>
<NameOfSubstance UI="D008031">Lignin</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>A1TA934AKO</RegistryNumber>
<NameOfSubstance UI="D014994">Xylose</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>EC 1.10.3.2</RegistryNumber>
<NameOfSubstance UI="D042845">Laccase</NameOfSubstance>
</Chemical>
</ChemicalList>
<CitationSubset>IM</CitationSubset>
<MeshHeadingList><MeshHeading><DescriptorName UI="D002473" MajorTopicYN="N">Cell Wall</DescriptorName>
<QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName>
<QualifierName UI="Q000201" MajorTopicYN="N">enzymology</QualifierName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D018506" MajorTopicYN="N">Gene Expression Regulation, Plant</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D042845" MajorTopicYN="N">Laccase</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D008031" MajorTopicYN="N">Lignin</DescriptorName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D030821" MajorTopicYN="N">Plants, Genetically Modified</DescriptorName>
<QualifierName UI="Q000201" MajorTopicYN="Y">enzymology</QualifierName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName>
<QualifierName UI="Q000201" MajorTopicYN="Y">enzymology</QualifierName>
<QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D014994" MajorTopicYN="N">Xylose</DescriptorName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
</MeshHeadingList>
<KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="Y">Populus</Keyword>
<Keyword MajorTopicYN="Y">biofuel</Keyword>
<Keyword MajorTopicYN="Y">cell wall</Keyword>
<Keyword MajorTopicYN="Y">lignin</Keyword>
<Keyword MajorTopicYN="Y">recalcitrance</Keyword>
<Keyword MajorTopicYN="Y">xylose</Keyword>
</KeywordList>
</MedlineCitation>
<PubmedData><History><PubMedPubDate PubStatus="received"><Year>2015</Year>
<Month>12</Month>
<Day>09</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="revised"><Year>2016</Year>
<Month>03</Month>
<Day>01</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="accepted"><Year>2016</Year>
<Month>03</Month>
<Day>12</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="entrez"><Year>2016</Year>
<Month>3</Month>
<Day>22</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="pubmed"><Year>2016</Year>
<Month>3</Month>
<Day>22</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="medline"><Year>2017</Year>
<Month>6</Month>
<Day>2</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
</History>
<PublicationStatus>ppublish</PublicationStatus>
<ArticleIdList><ArticleId IdType="pubmed">26997157</ArticleId>
<ArticleId IdType="pmc">PMC5043505</ArticleId>
<ArticleId IdType="doi">10.1111/pbi.12560</ArticleId>
</ArticleIdList>
<ReferenceList><Reference><Citation>Biotechnol Biofuels. 2013 Dec 16;6(1):183</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24341349</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Physiol. 2002 May;129(1):145-55</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12011346</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Annu Rev Chem Biomol Eng. 2011;2:121-45</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22432613</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Indian J Microbiol. 2008 Sep;48(3):309-16</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23100727</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Biotechnol Biofuels. 2012 Aug 11;5(1):58</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22883929</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>New Phytol. 2010 Jul;187(2):273-85</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20642725</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Science. 1993 Apr 30;260(5108):672-4</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17812228</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Methods. 2001 Dec;25(4):402-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">11846609</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>PLoS One. 2014 Aug 18;9(8):e105115</Citation>
<ArticleIdList><ArticleId IdType="pubmed">25133694</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Mol Biol Evol. 2011 Oct;28(10):2731-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21546353</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Proc Natl Acad Sci U S A. 2013 Jun 25;110(26):10848-53</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23754401</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Biomol Struct Dyn. 2015 Sep;33(9):1835-49</Citation>
<ArticleIdList><ArticleId IdType="pubmed">25301391</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Curr Opin Plant Biol. 2015 Jun;25:151-61</Citation>
<ArticleIdList><ArticleId IdType="pubmed">26051036</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Biotechnol Biofuels. 2012 Jan 04;5(1):2</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22214220</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Proc Natl Acad Sci U S A. 2011 Apr 12;108(15):6300-5</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21444820</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nat Methods. 2011 Sep 29;8(10):785-6</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21959131</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>FEMS Microbiol Rev. 2006 Mar;30(2):215-42</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16472305</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Planta. 2011 Mar;233(3):439-70</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21063888</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Bioresour Technol. 2016 Jan;200:761-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">26575618</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Exp Bot. 2006;57(11):2563-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16804053</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Curr Opin Biotechnol. 2012 Jun;23(3):330-7</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22209015</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nucleic Acids Res. 2012 Jan;40(Database issue):D1178-86</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22110026</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Cell. 2011 Mar;23(3):1124-37</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21447792</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Science. 2014 Apr 4;344(6179):90-3</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24700858</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Insect Biochem Mol Biol. 2010 Mar;40(3):179-88</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20219675</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Science. 2009 Apr 3;324(5923):89-91</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19342588</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nat Biotechnol. 2007 Jul;25(7):759-61</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17572667</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Biotechnol Biofuels. 2015 Mar 12;8:41</Citation>
<ArticleIdList><ArticleId IdType="pubmed">25802552</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Curr Opin Biotechnol. 2012 Jun;23(3):390-5</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22033175</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nucleic Acids Res. 2001 Jan 1;29(1):102-5</Citation>
<ArticleIdList><ArticleId IdType="pubmed">11125061</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Exp Bot. 2013 May;64(8):2449-66</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23599271</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Planta. 2005 Jul;221(5):619-36</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15940465</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Physiol. 1992 Jul;99(3):1162-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16668984</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Physiol. 2015 Apr;167(4):1284-95</Citation>
<ArticleIdList><ArticleId IdType="pubmed">25667313</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Eur J Biochem. 1999 Jan;259(1-2):485-95</Citation>
<ArticleIdList><ArticleId IdType="pubmed">9914531</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Arch Microbiol. 2003 Mar;179(3):145-50</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12610719</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Microbiology. 2013 Jul;159(Pt 7):1471-86</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23676433</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Cell. 2013 Oct;25(10):3976-87</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24143805</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Biotechnol J. 2015 May;13(4):514-25</Citation>
<ArticleIdList><ArticleId IdType="pubmed">25418842</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Insect Biochem Mol Biol. 2004 Jan;34(1):29-41</Citation>
<ArticleIdList><ArticleId IdType="pubmed">14723895</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Bioresour Technol. 2015 May;183:248-54</Citation>
<ArticleIdList><ArticleId IdType="pubmed">25746301</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Methods Mol Biol. 2009;581:169-83</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19768623</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
</PubmedData>
</pubmed>
<affiliations><list><country><li>États-Unis</li>
</country>
<region><li>Caroline du Sud</li>
<li>Colorado</li>
<li>Tennessee</li>
</region>
</list>
<tree><country name="États-Unis"><region name="Tennessee"><name sortKey="Bryan, Anthony C" sort="Bryan, Anthony C" uniqKey="Bryan A" first="Anthony C" last="Bryan">Anthony C. Bryan</name>
</region>
<name sortKey="Chen, Jin Gui" sort="Chen, Jin Gui" uniqKey="Chen J" first="Jin-Gui" last="Chen">Jin-Gui Chen</name>
<name sortKey="Collins, Cassandra M" sort="Collins, Cassandra M" uniqKey="Collins C" first="Cassandra M" last="Collins">Cassandra M. Collins</name>
<name sortKey="Engle, Nancy" sort="Engle, Nancy" uniqKey="Engle N" first="Nancy" last="Engle">Nancy Engle</name>
<name sortKey="Gjersing, Erica" sort="Gjersing, Erica" uniqKey="Gjersing E" first="Erica" last="Gjersing">Erica Gjersing</name>
<name sortKey="Gunter, Lee" sort="Gunter, Lee" uniqKey="Gunter L" first="Lee" last="Gunter">Lee Gunter</name>
<name sortKey="Hinchee, Maud A W" sort="Hinchee, Maud A W" uniqKey="Hinchee M" first="Maud A W" last="Hinchee">Maud A W. Hinchee</name>
<name sortKey="Jawdy, Sara" sort="Jawdy, Sara" uniqKey="Jawdy S" first="Sara" last="Jawdy">Sara Jawdy</name>
<name sortKey="Muchero, Wellington" sort="Muchero, Wellington" uniqKey="Muchero W" first="Wellington" last="Muchero">Wellington Muchero</name>
<name sortKey="Sykes, Robert" sort="Sykes, Robert" uniqKey="Sykes R" first="Robert" last="Sykes">Robert Sykes</name>
<name sortKey="Tschaplinski, Timothy J" sort="Tschaplinski, Timothy J" uniqKey="Tschaplinski T" first="Timothy J" last="Tschaplinski">Timothy J. Tschaplinski</name>
<name sortKey="Tuskan, Gerald A" sort="Tuskan, Gerald A" uniqKey="Tuskan G" first="Gerald A" last="Tuskan">Gerald A. Tuskan</name>
<name sortKey="Winkeler, Kimberly A" sort="Winkeler, Kimberly A" uniqKey="Winkeler K" first="Kimberly A" last="Winkeler">Kimberly A. Winkeler</name>
<name sortKey="Yang, Xiaohan" sort="Yang, Xiaohan" uniqKey="Yang X" first="Xiaohan" last="Yang">Xiaohan Yang</name>
</country>
</tree>
</affiliations>
</record>

Pour manipuler ce document sous Unix (Dilib)

EXPLOR_STEP=$WICRI_ROOT/Bois/explor/PoplarV1/Data/Main/Exploration

HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001800 | SxmlIndent | more

HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 001800 | SxmlIndent | more

Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Bois
   |area=    PoplarV1
   |flux=    Main
   |étape=   Exploration
   |type=    RBID
   |clé=     pubmed:26997157
   |texte=   Knockdown of a laccase in Populus deltoides confers altered cell wall chemistry and increased sugar release.
}}

Pour générer des pages wiki

HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i   -Sk "pubmed:26997157" \
       | HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd   \
       | NlmPubMed2Wicri -a PoplarV1

This area was generated with Dilib version V0.6.37.
Data generation: Wed Nov 18 12:07:19 2020. Site generation: Wed Nov 18 12:16:31 2020

	Serveur d'exploration sur le peuplier
	Attention, ce site est en cours de développement ! Attention, site généré par des moyens informatiques à partir de corpus bruts. Les informations ne sont donc pas validées.

Serveur d'exploration sur le peuplier

Knockdown of a laccase in Populus deltoides confers altered cell wall chemistry and increased sugar release.

Knockdown of a laccase in Populus deltoides confers altered cell wall chemistry and increased sugar release.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki